Pgem T Easy

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Pgem t easy

. This can be used with Gateway-compatible Homology-Directed Repair. It can be performed without the use of restriction enzymesIt is an easy and simple. PGEM-T Vector Information Description. Bacterial Expression Growth in Bacteria.

Video describing the use of the pGEM-T Vector Systems. PGEM-T Easy Vector Systemは従来のpGEM-T Vector Systemの機能に加えマルチクローニングサイトの両端にEcoRIとNotIサイトが加えられましたそのため1種類NotIEcoRIあるい. This vector is also known as pGEM5Zf. It is a sub cloning technique.

12μg pGEM-T easy vector 50ngμL 12μL control insert DNA 4ngμL 200μL rapid ligation Buffer 2X 100U T4 DNA Ligase JM109 12mL competent cells high efficiency 6 x 200μL Restriction Site. T-Overhangs for Easy PCR Cloning. Pgem T Easy Vector System Ii Cloning Kit supplied by Promega used in various techniques. An insert-to-vector ratio between 31 and 13 is recommended for the pGEM-T and pGEM-T Easy.

Li Qians lab is published in Sci Rep. TA cloning is also known as rapid cloning. Based on the order of amino acid encoding protein in recombinant plasmid pGEM-T Easy. The same amount of insert was used for every ligation regardless of TA vector used.

PGEM-T Easy linearized Linearized vector for TA cloning of PCR products. ZERO BIAS - scores article reviews protocol conditions. The pGEM -T and pGEM -T Easy Vector Systems are convenient systems for the cloning of PCR products. The pGEM-T ligations were increased to 12µl final volume to allow for a larger volume of insert.

Briefly centrifuge the pGEM -T or pGEM -T Easy. 99100 based on 1 PubMed citations. The MCS of the pGEM-T Easy Vector contains sequences on either side of the insert that are recognized by the. The insertion site is flanked by BstZI EcoRI and NotI sites.

Subscribe to Our Blog. Multiple Cloning Sequences Promega Corporation 2800 Woods Hollow Road Madison WI 53711-5399 USA Toll Free. Plasmid pGEMT-PTE2A from Dr. The cloning of MPT83 gene into pGEM-T Easy vector results in recombinant plasmid with the size of 3678 bp.

PGEM-T Parental vector for TA cloning of PCR products. Ad Get highly efficient PCR cloning kits ideal for both blunt-end and TA cloning. Gateway destination vector only containing the Gateway cassette and pGEM-T Easy backbone. The pGEM-T vector is a high-efficiency TA cloning vector which contains multiple cloning sites as shown below.

The pGEM -T and pGEM -T Easy Vectors are linearized vectors with a single 3-terminal thymidine at both ends. Order blunt-end and TA cloning vectors with TOP10 competent cells for subcloning. The pGEM-T vector is 30kb in size and contains the. The vectors are prepared by cutting the pGEM -5Zf and pGEM -T Easy Vectors respectively with EcoR V and adding a 3 terminal thymidine to both ends.

The only difference between pGEM-T and pGEM-T Easy is in the multiple cloning site MCS. Promega Backbone size wo insert bp 3015 Vector type. The insertion site is flanked by BstZI sites. Ligation Using 2X Rapid Ligation Buffer 1.

Quick PROTOCOL 1 pGEM-T and pGEM-T Easy Vector Systems INSTRUCTIONS FOR USE OF PRODUCTS A1360 A1380 A3600 AND A3610. Learn about the latest plasmid. The T-overhangs at the insertion site greatly improve the. PGEM -T and pGEM -T Easy Vector Multiple Cloning Sequences and Maps IIA.

BstZI NotI and EcoRI. PGEM-T Easy Search Vector Database Backbone manufacturer. Order blunt-end and TA cloning vectors with TOP10 competent cells for subcloning. This plasmid is available through Addgene.

Ad Get highly efficient PCR cloning kits ideal for both blunt-end and TA cloning.

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